Journal: Dentistry Journal

Article Title: Effects of miR-128-3p on Renal Inflammation in a Rat Periodontitis Model

doi: 10.3390/dj13120577

Figure Lengend Snippet: Representative image of characteristics of EVs after isolating them from plasma. ( a ) Representative immunogold TEM results of plasma EVs ( b ) Primary antibody marker CD9 attached to the EVs identified in TEM ( c ) Presence of CD63 marker attached to the EVs ( d ) NTA analysis of plasma EVs.

Article Snippet: The NTA analysis software (Zetasizer Nano software v3.30; Malvern Panalytical) was used for further analyzing the nanoparticle tracking data based on the videos of completed tracks.

Techniques: Clinical Proteomics, Marker

Journal: Molecular cell

Article Title: Cancer cells employ nuclear caspase-8 to overcome the p53-dependent G2/M checkpoint through cleavage of USP28

doi: 10.1016/j.molcel.2019.12.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: After blocking with 5% FCS in PBS (++) for 30 min at RT, primary antibodies against caspase-8 (#PA5-21336, Thermo Scientific), cleaved caspase-8 (#sc-166320; Santa Cruz), and myc-tag (#ab18185, Abcam), respectively, were incubated for at RT 2 h. After washing, Alexa-488 or −546 coupled isotype-specific secondary antibodies (#A11034, #A11030, Life Technologies) were incubated at RT for 1 h, coverslips washed, and mounted in Mowiol (#D2522, Sigma).

Techniques: Western Blot, Immunocytochemistry, Immunohistochemistry, Recombinant, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Extraction, Imaging, Software